| [Plate: M 410 #1] | ||||||||||||
| Time=00:00:00 | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.053 | 0.058 | 0.057 | 0.034 | 0.037 | 0.034 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 |
| B | 0.054 | 0.057 | 0.055 | 0.034 | 0.034 | 0.035 | 0.034 | 0.034 | 0.035 | 0.034 | 0.034 | 0.034 |
| C | 0.054 | 0.062 | 0.055 | 0.037 | 0.035 | 0.036 | 0.034 | 0.035 | 0.035 | 0.033 | 0.035 | 0.033 |
| D | 0.06 | 0.065 | 0.061 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 |
| E | 0.054 | 0.061 | 0.06 | 0.034 | 0.035 | 0.034 | 0.034 | 0.035 | 0.034 | 0.037 | 0.034 | 0.034 |
| F | 0.054 | 0.062 | 0.065 | 0.034 | 0.034 | 0.037 | 0.036 | 0.034 | 0.034 | 0.035 | 0.034 | 0.033 |
| G | 0.059 | 0.064 | 0.06 | 0.038 | 0.037 | 0.036 | 0.035 | 0.034 | 0.034 | 0.039 | 0.035 | 0.034 |
| H | 0.06 | 0.062 | 0.063 | 0.035 | 0.039 | 0.035 | 0.036 | 0.035 | 0.036 | 0.035 | 0.035 | 0.035 |
| [Plate: M 410 #2] | ||||||||||||
| Time=00:01:00 | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.065 | 0.074 | 0.073 | 0.035 | 0.037 | 0.034 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 |
| B | 0.069 | 0.079 | 0.072 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 | 0.035 | 0.034 | 0.035 |
| C | 0.069 | 0.078 | 0.073 | 0.037 | 0.035 | 0.036 | 0.034 | 0.035 | 0.036 | 0.034 | 0.035 | 0.033 |
| D | 0.076 | 0.081 | 0.076 | 0.035 | 0.036 | 0.035 | 0.035 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 |
| E | 0.071 | 0.079 | 0.078 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 | 0.035 | 0.037 | 0.034 | 0.035 |
| F | 0.068 | 0.079 | 0.08 | 0.034 | 0.035 | 0.037 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 |
| G | 0.073 | 0.074 | 0.073 | 0.038 | 0.037 | 0.037 | 0.035 | 0.035 | 0.035 | 0.039 | 0.036 | 0.034 |
| H | 0.074 | 0.078 | 0.08 | 0.036 | 0.039 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.035 |
| [Plate: M 410 #3] | ||||||||||||
| Time=00:02:00 | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.079 | 0.089 | 0.087 | 0.035 | 0.037 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 |
| B | 0.083 | 0.095 | 0.088 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 | 0.035 | 0.034 | 0.035 |
| C | 0.084 | 0.093 | 0.088 | 0.037 | 0.035 | 0.036 | 0.034 | 0.035 | 0.036 | 0.034 | 0.035 | 0.033 |
| D | 0.089 | 0.096 | 0.094 | 0.035 | 0.036 | 0.035 | 0.035 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 |
| E | 0.085 | 0.097 | 0.095 | 0.035 | 0.035 | 0.034 | 0.034 | 0.035 | 0.035 | 0.037 | 0.034 | 0.035 |
| F | 0.083 | 0.097 | 0.094 | 0.034 | 0.035 | 0.037 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 |
| G | 0.089 | 0.09 | 0.088 | 0.039 | 0.037 | 0.037 | 0.035 | 0.035 | 0.035 | 0.039 | 0.035 | 0.035 |
| H | 0.089 | 0.096 | 0.095 | 0.036 | 0.039 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.035 |
| [Plate: M 410 #4] | ||||||||||||
| Time=00:03:00 | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.094 | 0.104 | 0.102 | 0.035 | 0.038 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 |
| B | 0.097 | 0.111 | 0.103 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 | 0.035 | 0.034 | 0.035 |
| C | 0.099 | 0.109 | 0.105 | 0.037 | 0.035 | 0.036 | 0.035 | 0.035 | 0.036 | 0.034 | 0.035 | 0.034 |
| D | 0.105 | 0.111 | 0.104 | 0.036 | 0.036 | 0.035 | 0.035 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 |
| E | 0.1 | 0.113 | 0.112 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.037 | 0.035 | 0.035 |
| F | 0.097 | 0.112 | 0.108 | 0.034 | 0.035 | 0.038 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 |
| G | 0.106 | 0.106 | 0.104 | 0.039 | 0.037 | 0.037 | 0.035 | 0.035 | 0.035 | 0.039 | 0.036 | 0.035 |
| H | 0.103 | 0.108 | 0.111 | 0.036 | 0.039 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.035 |
| [Plate: M 410 #5] | ||||||||||||
| Time=00:04:00 | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.106 | 0.119 | 0.115 | 0.035 | 0.038 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 |
| B | 0.111 | 0.126 | 0.119 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 | 0.035 | 0.035 | 0.034 | 0.035 |
| C | 0.114 | 0.123 | 0.12 | 0.037 | 0.035 | 0.036 | 0.035 | 0.035 | 0.036 | 0.034 | 0.035 | 0.033 |
| D | 0.118 | 0.125 | 0.12 | 0.036 | 0.036 | 0.035 | 0.035 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 |
| E | 0.115 | 0.128 | 0.128 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.035 | 0.038 | 0.034 | 0.035 |
| F | 0.111 | 0.127 | 0.122 | 0.034 | 0.035 | 0.038 | 0.036 | 0.035 | 0.035 | 0.035 | 0.035 | 0.034 |
| G | 0.121 | 0.121 | 0.119 | 0.039 | 0.037 | 0.037 | 0.035 | 0.035 | 0.035 | 0.039 | 0.036 | 0.035 |
| H | 0.116 | 0.123 | 0.125 | 0.036 | 0.039 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.036 | 0.035 |
| Initial | final | Difference | ||||||||||
| reading | reading | (5 min - | ||||||||||
| (time 0) | (5 min) | 0 min) | ||||||||||
| 0.053 | 0.106 | 0.053 | The time between when you added substrate to your | |||||||||
| 0.054 | 0.111 | 0.057 | plate and when we put the plate into the machine may | |||||||||
| 0.054 | 0.114 | 0.060 | have varied for each run (each pH we tested). So we | |||||||||
| 0.060 | 0.118 | 0.058 | subtract the initial reading from the final reading to | |||||||||
| 0.054 | 0.115 | 0.061 | determine how much absorbance is due to the enzyme | |||||||||
| 0.054 | 0.111 | 0.057 | converting S --> P in exactly 5 min. | |||||||||
| 0.059 | 0.121 | 0.062 | ||||||||||
| 0.060 | 0.116 | 0.056 | Then I used excel to calculate the average difference | |||||||||
| 0.058 | 0.119 | 0.061 | in absorbance: .061, and a standard deviation | |||||||||
| 0.057 | 0.126 | 0.069 | (look in 'Help' for STDEV to see which formula I used) | |||||||||
| 0.062 | 0.123 | 0.061 | ||||||||||
| 0.065 | 0.125 | 0.060 | ||||||||||
| 0.061 | 0.128 | 0.067 | ||||||||||
| 0.062 | 0.127 | 0.065 | ||||||||||
| 0.064 | 0.121 | 0.057 | ||||||||||
| 0.062 | 0.123 | 0.061 | ||||||||||
| 0.057 | 0.115 | 0.058 | ||||||||||
| 0.055 | 0.119 | 0.064 | ||||||||||
| 0.055 | 0.120 | 0.065 | ||||||||||
| 0.061 | 0.120 | 0.059 | ||||||||||
| 0.060 | 0.128 | 0.068 | ||||||||||
| 0.065 | 0.122 | 0.057 | ||||||||||
| 0.060 | 0.119 | 0.059 | ||||||||||
| 0.063 | 0.125 | 0.062 | ||||||||||
| 0.061 | mean | |||||||||||
| 0.004 | std dev | |||||||||||