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[Plate: M 410 #1] |
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Time=00:00:00 |
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
0.053 |
0.058 |
0.057 |
0.034 |
0.037 |
0.034 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
B |
0.054 |
0.057 |
0.055 |
0.034 |
0.034 |
0.035 |
0.034 |
0.034 |
0.035 |
0.034 |
0.034 |
0.034 |
C |
0.054 |
0.062 |
0.055 |
0.037 |
0.035 |
0.036 |
0.034 |
0.035 |
0.035 |
0.033 |
0.035 |
0.033 |
D |
0.06 |
0.065 |
0.061 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
E |
0.054 |
0.061 |
0.06 |
0.034 |
0.035 |
0.034 |
0.034 |
0.035 |
0.034 |
0.037 |
0.034 |
0.034 |
F |
0.054 |
0.062 |
0.065 |
0.034 |
0.034 |
0.037 |
0.036 |
0.034 |
0.034 |
0.035 |
0.034 |
0.033 |
G |
0.059 |
0.064 |
0.06 |
0.038 |
0.037 |
0.036 |
0.035 |
0.034 |
0.034 |
0.039 |
0.035 |
0.034 |
H |
0.06 |
0.062 |
0.063 |
0.035 |
0.039 |
0.035 |
0.036 |
0.035 |
0.036 |
0.035 |
0.035 |
0.035 |
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[Plate: M 410 #2] |
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Time=00:01:00 |
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
0.065 |
0.074 |
0.073 |
0.035 |
0.037 |
0.034 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
B |
0.069 |
0.079 |
0.072 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
0.035 |
0.034 |
0.035 |
C |
0.069 |
0.078 |
0.073 |
0.037 |
0.035 |
0.036 |
0.034 |
0.035 |
0.036 |
0.034 |
0.035 |
0.033 |
D |
0.076 |
0.081 |
0.076 |
0.035 |
0.036 |
0.035 |
0.035 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
E |
0.071 |
0.079 |
0.078 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
0.035 |
0.037 |
0.034 |
0.035 |
F |
0.068 |
0.079 |
0.08 |
0.034 |
0.035 |
0.037 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
G |
0.073 |
0.074 |
0.073 |
0.038 |
0.037 |
0.037 |
0.035 |
0.035 |
0.035 |
0.039 |
0.036 |
0.034 |
H |
0.074 |
0.078 |
0.08 |
0.036 |
0.039 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.035 |
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[Plate: M 410 #3] |
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Time=00:02:00 |
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
0.079 |
0.089 |
0.087 |
0.035 |
0.037 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
B |
0.083 |
0.095 |
0.088 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
0.035 |
0.034 |
0.035 |
C |
0.084 |
0.093 |
0.088 |
0.037 |
0.035 |
0.036 |
0.034 |
0.035 |
0.036 |
0.034 |
0.035 |
0.033 |
D |
0.089 |
0.096 |
0.094 |
0.035 |
0.036 |
0.035 |
0.035 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
E |
0.085 |
0.097 |
0.095 |
0.035 |
0.035 |
0.034 |
0.034 |
0.035 |
0.035 |
0.037 |
0.034 |
0.035 |
F |
0.083 |
0.097 |
0.094 |
0.034 |
0.035 |
0.037 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
G |
0.089 |
0.09 |
0.088 |
0.039 |
0.037 |
0.037 |
0.035 |
0.035 |
0.035 |
0.039 |
0.035 |
0.035 |
H |
0.089 |
0.096 |
0.095 |
0.036 |
0.039 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.035 |
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[Plate: M 410 #4] |
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Time=00:03:00 |
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
0.094 |
0.104 |
0.102 |
0.035 |
0.038 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
B |
0.097 |
0.111 |
0.103 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
0.035 |
0.034 |
0.035 |
C |
0.099 |
0.109 |
0.105 |
0.037 |
0.035 |
0.036 |
0.035 |
0.035 |
0.036 |
0.034 |
0.035 |
0.034 |
D |
0.105 |
0.111 |
0.104 |
0.036 |
0.036 |
0.035 |
0.035 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
E |
0.1 |
0.113 |
0.112 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.037 |
0.035 |
0.035 |
F |
0.097 |
0.112 |
0.108 |
0.034 |
0.035 |
0.038 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
G |
0.106 |
0.106 |
0.104 |
0.039 |
0.037 |
0.037 |
0.035 |
0.035 |
0.035 |
0.039 |
0.036 |
0.035 |
H |
0.103 |
0.108 |
0.111 |
0.036 |
0.039 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.035 |
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[Plate: M 410 #5] |
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Time=00:04:00 |
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
0.106 |
0.119 |
0.115 |
0.035 |
0.038 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
B |
0.111 |
0.126 |
0.119 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
0.035 |
0.035 |
0.034 |
0.035 |
C |
0.114 |
0.123 |
0.12 |
0.037 |
0.035 |
0.036 |
0.035 |
0.035 |
0.036 |
0.034 |
0.035 |
0.033 |
D |
0.118 |
0.125 |
0.12 |
0.036 |
0.036 |
0.035 |
0.035 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
E |
0.115 |
0.128 |
0.128 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.035 |
0.038 |
0.034 |
0.035 |
F |
0.111 |
0.127 |
0.122 |
0.034 |
0.035 |
0.038 |
0.036 |
0.035 |
0.035 |
0.035 |
0.035 |
0.034 |
G |
0.121 |
0.121 |
0.119 |
0.039 |
0.037 |
0.037 |
0.035 |
0.035 |
0.035 |
0.039 |
0.036 |
0.035 |
H |
0.116 |
0.123 |
0.125 |
0.036 |
0.039 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.036 |
0.035 |
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Initial |
final |
Difference |
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reading |
reading |
(5 min - |
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(time 0) |
(5 min) |
0 min) |
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0.053 |
0.106 |
0.053 |
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The time between when you added substrate to your |
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0.054 |
0.111 |
0.057 |
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plate and when we put the plate into the machine may |
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0.054 |
0.114 |
0.060 |
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have varied for each run (each pH we tested). So we |
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0.060 |
0.118 |
0.058 |
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subtract the initial reading from the final reading to |
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0.054 |
0.115 |
0.061 |
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determine how much absorbance is due to the enzyme |
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0.054 |
0.111 |
0.057 |
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converting S --> P in exactly 5 min. |
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0.059 |
0.121 |
0.062 |
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0.060 |
0.116 |
0.056 |
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Then I used excel to calculate the average difference |
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0.058 |
0.119 |
0.061 |
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in absorbance: .061, and a standard deviation |
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0.057 |
0.126 |
0.069 |
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(look in 'Help' for STDEV to see which formula I used) |
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0.062 |
0.123 |
0.061 |
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0.065 |
0.125 |
0.060 |
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0.061 |
0.128 |
0.067 |
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0.062 |
0.127 |
0.065 |
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0.064 |
0.121 |
0.057 |
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0.062 |
0.123 |
0.061 |
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0.057 |
0.115 |
0.058 |
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0.055 |
0.119 |
0.064 |
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0.055 |
0.120 |
0.065 |
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0.061 |
0.120 |
0.059 |
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0.060 |
0.128 |
0.068 |
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0.065 |
0.122 |
0.057 |
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0.060 |
0.119 |
0.059 |
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0.063 |
0.125 |
0.062 |
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0.061 |
mean |
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0.004 |
std dev |
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